产品货号：
Z15342
中文名称：
CYP7A1抗体
英文名称：
Anti-CYP7A1 Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-CYP7A1 Antibody |
| 指标别名 | cytochrome P450 family 7 subfamily A member 1;CP7A;CYP7;CYPVII;CYP7A1 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | WB,IHC,ICC/IF,FCM |
| 基因名称 | CYP7A1 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human CYP7A1 recombinant protein (Position:I10-L504). |
| 实际分子量 | 58KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | CYP7A1(Cytochrome P450 Subfamily VIIA Polypeptide 1),also called CYP7 or CHOLESTEROL 7-ALPHA-HYDROXYLASE,is an enzyme that in humans is encoded by the CYP7A1 gene.Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization,Cohen et al.(1992) mapped the CYP7 gene to 8q11-q12.By transfection of reporter constructs,mutation analysis,and DNase footprinting,Molowa et al.(1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation.They found HNF3 to be an activator of CYP7A1 activity.Agellon et al.(2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding.Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice,but repressed human CYP7A1 mRNA and activity in transgenic mice. |
| Uniprot ID | P22680 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | cytochrome P450 family 7 subfamily A member 1 |
| 蛋白名全称 | Cytochrome P450 7A1 |
| 推荐稀释比 | Western blot(WB) 1:500～2000 Flow cytometry (FCM) 1～3μg/1×106 cells Immunocytochemistry/Immunofluorescence(ICC/IF) 1:50～400 Immunohistochemistry in paraffin section (IHC) 1:50～400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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